mouse vcam Search Results


invivo mab anti mouse cd106 vcam 1  (Bio X Cell)
94
Bio X Cell invivo mab anti mouse cd106 vcam 1
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Invivo Mab Anti Mouse Cd106 Vcam 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/invivo mab anti mouse cd106 vcam 1/product/Bio X Cell
Average 94 stars, based on 1 article reviews
invivo mab anti mouse cd106 vcam 1 - by Bioz Stars, 2026-04
94/100 stars
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mouse vcam 1 cd106 mab  (R&D Systems)
90
R&D Systems mouse vcam 1 cd106 mab
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Mouse Vcam 1 Cd106 Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse vcam 1 cd106 mab/product/R&D Systems
Average 90 stars, based on 1 article reviews
mouse vcam 1 cd106 mab - by Bioz Stars, 2026-04
90/100 stars
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recombinant mouse vcam  (R&D Systems)
94
R&D Systems recombinant mouse vcam
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Recombinant Mouse Vcam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant mouse vcam/product/R&D Systems
Average 94 stars, based on 1 article reviews
recombinant mouse vcam - by Bioz Stars, 2026-04
94/100 stars
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standard elisa kit  (R&D Systems)
94
R&D Systems standard elisa kit
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Standard Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard elisa kit/product/R&D Systems
Average 94 stars, based on 1 article reviews
standard elisa kit - by Bioz Stars, 2026-04
94/100 stars
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mouse vcam  (R&D Systems)
94
R&D Systems mouse vcam
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Mouse Vcam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse vcam/product/R&D Systems
Average 94 stars, based on 1 article reviews
mouse vcam - by Bioz Stars, 2026-04
94/100 stars
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af643 von willebrand factor rabbit polyclonal  (R&D Systems)
93
R&D Systems af643 von willebrand factor rabbit polyclonal
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Af643 Von Willebrand Factor Rabbit Polyclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af643 von willebrand factor rabbit polyclonal/product/R&D Systems
Average 93 stars, based on 1 article reviews
af643 von willebrand factor rabbit polyclonal - by Bioz Stars, 2026-04
93/100 stars
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svcam 1 mouse svcam 1 cd106 elisa kit  (R&D Systems Hematology)
94
R&D Systems Hematology svcam 1 mouse svcam 1 cd106 elisa kit
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Svcam 1 Mouse Svcam 1 Cd106 Elisa Kit, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/svcam 1 mouse svcam 1 cd106 elisa kit/product/R&D Systems Hematology
Average 94 stars, based on 1 article reviews
svcam 1 mouse svcam 1 cd106 elisa kit - by Bioz Stars, 2026-04
94/100 stars
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quantikine elisa kit  (R&D Systems)
93
R&D Systems quantikine elisa kit
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/quantikine elisa kit/product/R&D Systems
Average 93 stars, based on 1 article reviews
quantikine elisa kit - by Bioz Stars, 2026-04
93/100 stars
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anti vcam 1 cd106  (R&D Systems)
93
R&D Systems anti vcam 1 cd106
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Anti Vcam 1 Cd106, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti vcam 1 cd106/product/R&D Systems
Average 93 stars, based on 1 article reviews
anti vcam 1 cd106 - by Bioz Stars, 2026-04
93/100 stars
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cd106 antibody af643  (R&D Systems)
94
R&D Systems cd106 antibody af643
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Cd106 Antibody Af643, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd106 antibody af643/product/R&D Systems
Average 94 stars, based on 1 article reviews
cd106 antibody af643 - by Bioz Stars, 2026-04
94/100 stars
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vcam 1  (R&D Systems)
93
R&D Systems vcam 1
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Vcam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vcam 1/product/R&D Systems
Average 93 stars, based on 1 article reviews
vcam 1 - by Bioz Stars, 2026-04
93/100 stars
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rmvcam 1 fc chimera  (R&D Systems)
94
R&D Systems rmvcam 1 fc chimera
Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for <t>CD106</t> + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Rmvcam 1 Fc Chimera, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rmvcam 1 fc chimera/product/R&D Systems
Average 94 stars, based on 1 article reviews
rmvcam 1 fc chimera - by Bioz Stars, 2026-04
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Image Search Results


Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for CD106 + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.

Journal: EMBO Reports

Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer

doi: 10.15252/embr.202256524

Figure Lengend Snippet: Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for CD106 + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.

Article Snippet: InVivo Mab anti‐mouse CD106 (VCAM‐1) , Bio X Cell , Cat# BE0027, RRID:AB_1107572.

Techniques: Immunohistochemistry, Immunofluorescence, Staining

Representative IHC staining for CD106 + in normal pancreas. Scale bar, 50 μm. Representative IHC staining for CD106 + in IPMN+PanIN I‐III and IPMT human samples. The adjacent region (above) and tumor core (below) were paired from the same patient respectively, corresponding to the diagnosis. The red arrow indicated the CD106 + spindle‐like cells. Scale bar, 50 μm. Representative IHC staining of pancreatic lesions of human TMA for CD106 + . The magnification region indicates representative CD106 + cells. Scale bar, 200, 50 μm. Quantification of CD106 expression in pancreatitis, PanIN and PDAC lesions according to patients' pathological references of TMA (Appendix Table ). * P < 0.05 and ** P < 0.01. **** P < 0.0001. The P ‐values were calculated using Student's t ‐test. Representative IF staining of the PeSC cell line on a coverslip for CD106 (green), mCherry (red), and DAPI (blue). Scale bar, 50 μm.

Journal: EMBO Reports

Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer

doi: 10.15252/embr.202256524

Figure Lengend Snippet: Representative IHC staining for CD106 + in normal pancreas. Scale bar, 50 μm. Representative IHC staining for CD106 + in IPMN+PanIN I‐III and IPMT human samples. The adjacent region (above) and tumor core (below) were paired from the same patient respectively, corresponding to the diagnosis. The red arrow indicated the CD106 + spindle‐like cells. Scale bar, 50 μm. Representative IHC staining of pancreatic lesions of human TMA for CD106 + . The magnification region indicates representative CD106 + cells. Scale bar, 200, 50 μm. Quantification of CD106 expression in pancreatitis, PanIN and PDAC lesions according to patients' pathological references of TMA (Appendix Table ). * P < 0.05 and ** P < 0.01. **** P < 0.0001. The P ‐values were calculated using Student's t ‐test. Representative IF staining of the PeSC cell line on a coverslip for CD106 (green), mCherry (red), and DAPI (blue). Scale bar, 50 μm.

Article Snippet: InVivo Mab anti‐mouse CD106 (VCAM‐1) , Bio X Cell , Cat# BE0027, RRID:AB_1107572.

Techniques: Immunohistochemistry, Biomarker Discovery, Expressing, Staining

Experimental protocol for bone marrow chimeras. One month old KC mice were sublethally irradiated and reconstituted with bone marrow cells from a Tomato expressing mouse. After reconstitution, the chimeras were sacrificed on day 60. Representative dot plots of reconstituted bone marrow cells (Tomato + ) in KC chimeras compared to the nonreconstituted KC mice as control by FACS analysis. Quantification of reconstituted cells (Tomato + ) among CD45 + immune cells in either pancreas or periphery of KC chimeras compared to the control. Three mice in each condition. Representative dot plots and gating strategy of distinguishing the reconstituted CD106 + cells (Tomato + ) and nonreconstituted CD106 + cells (Tomato − ) in KC chimeras. Three mice in the group, each dot represents one mouse. Representative IF staining for CD106 (green) and Tomato (red) in the pancreas of chimera mice. Inset shows colocalization of CD106‐ and Tomato‐stained cells. Scale bar, 50 μm (upper), 10 μm (lower). Data information: ns not significant, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. The P ‐values were calculated using Student's t ‐test.

Journal: EMBO Reports

Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer

doi: 10.15252/embr.202256524

Figure Lengend Snippet: Experimental protocol for bone marrow chimeras. One month old KC mice were sublethally irradiated and reconstituted with bone marrow cells from a Tomato expressing mouse. After reconstitution, the chimeras were sacrificed on day 60. Representative dot plots of reconstituted bone marrow cells (Tomato + ) in KC chimeras compared to the nonreconstituted KC mice as control by FACS analysis. Quantification of reconstituted cells (Tomato + ) among CD45 + immune cells in either pancreas or periphery of KC chimeras compared to the control. Three mice in each condition. Representative dot plots and gating strategy of distinguishing the reconstituted CD106 + cells (Tomato + ) and nonreconstituted CD106 + cells (Tomato − ) in KC chimeras. Three mice in the group, each dot represents one mouse. Representative IF staining for CD106 (green) and Tomato (red) in the pancreas of chimera mice. Inset shows colocalization of CD106‐ and Tomato‐stained cells. Scale bar, 50 μm (upper), 10 μm (lower). Data information: ns not significant, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. The P ‐values were calculated using Student's t ‐test.

Article Snippet: InVivo Mab anti‐mouse CD106 (VCAM‐1) , Bio X Cell , Cat# BE0027, RRID:AB_1107572.

Techniques: Irradiation, Expressing, Control, Staining

A, B In vivo neutralization of CD106: (A) Experiment setting. (B) Tumor weight, FACS analysis of % PeSC + among CD45 − cells, % Epi + among CD45 − cells, % CD11b + among CD45 + cells, % Ly6G + CD11b + among CD45 + cells, % CD11b + F4/80 + among CD45 + cells, % CD11b + F4/80 + CD103 + among CD45 + cells (eight mice in each group, five for FACS analysis, and three for histological analysis. Quantification of PeSCs and Epi cells were cumulative from two FACS panels as technical duplicates). C–L In vivo depletion of CCL5 impact the macrophages differentiation: (C) Experimental setting. (D) Tumor weight of the grafts formed by Epi + PeSC in CCL5‐depleted conditions compared with undepleted conditions. FACS analysis of the percentage of CD11b + cells (E), Ly6G + CD11b + (F), F4/80 + CD11b + (G), F4/80 + CSFR1 + (H), F4/80 + CD163 + (I), F4/80 + CD11b + CD103 + (J), F4/80 + CD11b + PD‐L1 + (K) and CD8 + cells (L) among CD45 + cells derived from the tumor grafts. Ten mice in each group, six for FACS analysis and four for histological analysis. Data information: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The P ‐values were calculated using Student's t ‐test.

Journal: EMBO Reports

Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer

doi: 10.15252/embr.202256524

Figure Lengend Snippet: A, B In vivo neutralization of CD106: (A) Experiment setting. (B) Tumor weight, FACS analysis of % PeSC + among CD45 − cells, % Epi + among CD45 − cells, % CD11b + among CD45 + cells, % Ly6G + CD11b + among CD45 + cells, % CD11b + F4/80 + among CD45 + cells, % CD11b + F4/80 + CD103 + among CD45 + cells (eight mice in each group, five for FACS analysis, and three for histological analysis. Quantification of PeSCs and Epi cells were cumulative from two FACS panels as technical duplicates). C–L In vivo depletion of CCL5 impact the macrophages differentiation: (C) Experimental setting. (D) Tumor weight of the grafts formed by Epi + PeSC in CCL5‐depleted conditions compared with undepleted conditions. FACS analysis of the percentage of CD11b + cells (E), Ly6G + CD11b + (F), F4/80 + CD11b + (G), F4/80 + CSFR1 + (H), F4/80 + CD163 + (I), F4/80 + CD11b + CD103 + (J), F4/80 + CD11b + PD‐L1 + (K) and CD8 + cells (L) among CD45 + cells derived from the tumor grafts. Ten mice in each group, six for FACS analysis and four for histological analysis. Data information: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The P ‐values were calculated using Student's t ‐test.

Article Snippet: InVivo Mab anti‐mouse CD106 (VCAM‐1) , Bio X Cell , Cat# BE0027, RRID:AB_1107572.

Techniques: In Vivo, Neutralization, Derivative Assay

Journal: EMBO Reports

Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer

doi: 10.15252/embr.202256524

Figure Lengend Snippet:

Article Snippet: InVivo Mab anti‐mouse CD106 (VCAM‐1) , Bio X Cell , Cat# BE0027, RRID:AB_1107572.

Techniques: Recombinant, Purification, Plasmid Preparation, Control, Mutagenesis, Sequencing, SYBR Green Assay, Saline, Modification, Membrane, Activation Assay, Concentration Assay, Staining, Western Blot