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Image Search Results
Journal: EMBO Reports
Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
doi: 10.15252/embr.202256524
Figure Lengend Snippet: Representative IHC staining of tumor core and adjacent region in PDAC patient (stage II) for CD106 + . The corresponding magnification insets of the tumor core (red dotted line) or adjacent region (green dotted line) are displayed on the right. Scale bar, 500 μm in gross, 20 μm in magnification. Immunofluorescence staining of the pancreas obtained from a 2.5‐month‐old KC mouse for CD106 (red), α‐SMA (green) or CK19 (green), and DAPI (blue). Scale bar, 50 μm, 10 μm. Gating strategy for the identification of the CD106 + CD24 + CD44 + population in KC and WT mice. FACS analysis of the percentage of CD106 + among CD45 − CD31 − cells; each dot represents one mouse. The bars are representing the mean. The P ‐values were calculated using Student's t ‐test. Immunofluorescence staining of pancreases obtained from a KC mouse (left) and a 2.5‐month‐old KPC mouse (right) for CD44 (green), CD24 (red), and DAPI (blue). Scale bar, 50, 10 μm. Source data are available online for this figure.
Article Snippet:
Techniques: Immunohistochemistry, Immunofluorescence, Staining
Journal: EMBO Reports
Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
doi: 10.15252/embr.202256524
Figure Lengend Snippet: Representative IHC staining for CD106 + in normal pancreas. Scale bar, 50 μm. Representative IHC staining for CD106 + in IPMN+PanIN I‐III and IPMT human samples. The adjacent region (above) and tumor core (below) were paired from the same patient respectively, corresponding to the diagnosis. The red arrow indicated the CD106 + spindle‐like cells. Scale bar, 50 μm. Representative IHC staining of pancreatic lesions of human TMA for CD106 + . The magnification region indicates representative CD106 + cells. Scale bar, 200, 50 μm. Quantification of CD106 expression in pancreatitis, PanIN and PDAC lesions according to patients' pathological references of TMA (Appendix Table ). * P < 0.05 and ** P < 0.01. **** P < 0.0001. The P ‐values were calculated using Student's t ‐test. Representative IF staining of the PeSC cell line on a coverslip for CD106 (green), mCherry (red), and DAPI (blue). Scale bar, 50 μm.
Article Snippet:
Techniques: Immunohistochemistry, Biomarker Discovery, Expressing, Staining
Journal: EMBO Reports
Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
doi: 10.15252/embr.202256524
Figure Lengend Snippet: Experimental protocol for bone marrow chimeras. One month old KC mice were sublethally irradiated and reconstituted with bone marrow cells from a Tomato expressing mouse. After reconstitution, the chimeras were sacrificed on day 60. Representative dot plots of reconstituted bone marrow cells (Tomato + ) in KC chimeras compared to the nonreconstituted KC mice as control by FACS analysis. Quantification of reconstituted cells (Tomato + ) among CD45 + immune cells in either pancreas or periphery of KC chimeras compared to the control. Three mice in each condition. Representative dot plots and gating strategy of distinguishing the reconstituted CD106 + cells (Tomato + ) and nonreconstituted CD106 + cells (Tomato − ) in KC chimeras. Three mice in the group, each dot represents one mouse. Representative IF staining for CD106 (green) and Tomato (red) in the pancreas of chimera mice. Inset shows colocalization of CD106‐ and Tomato‐stained cells. Scale bar, 50 μm (upper), 10 μm (lower). Data information: ns not significant, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. The P ‐values were calculated using Student's t ‐test.
Article Snippet:
Techniques: Irradiation, Expressing, Control, Staining
Journal: EMBO Reports
Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
doi: 10.15252/embr.202256524
Figure Lengend Snippet: A, B In vivo neutralization of CD106: (A) Experiment setting. (B) Tumor weight, FACS analysis of % PeSC + among CD45 − cells, % Epi + among CD45 − cells, % CD11b + among CD45 + cells, % Ly6G + CD11b + among CD45 + cells, % CD11b + F4/80 + among CD45 + cells, % CD11b + F4/80 + CD103 + among CD45 + cells (eight mice in each group, five for FACS analysis, and three for histological analysis. Quantification of PeSCs and Epi cells were cumulative from two FACS panels as technical duplicates). C–L In vivo depletion of CCL5 impact the macrophages differentiation: (C) Experimental setting. (D) Tumor weight of the grafts formed by Epi + PeSC in CCL5‐depleted conditions compared with undepleted conditions. FACS analysis of the percentage of CD11b + cells (E), Ly6G + CD11b + (F), F4/80 + CD11b + (G), F4/80 + CSFR1 + (H), F4/80 + CD163 + (I), F4/80 + CD11b + CD103 + (J), F4/80 + CD11b + PD‐L1 + (K) and CD8 + cells (L) among CD45 + cells derived from the tumor grafts. Ten mice in each group, six for FACS analysis and four for histological analysis. Data information: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. The P ‐values were calculated using Student's t ‐test.
Article Snippet:
Techniques: In Vivo, Neutralization, Derivative Assay
Journal: EMBO Reports
Article Title: Pericyte stem cells induce Ly6G + cell accumulation and immunotherapy resistance in pancreatic cancer
doi: 10.15252/embr.202256524
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Purification, Plasmid Preparation, Control, Mutagenesis, Sequencing, SYBR Green Assay, Saline, Modification, Membrane, Activation Assay, Concentration Assay, Staining, Western Blot
Journal: Neural Regeneration Research
Article Title: ADAM10 facilitates rapid neural stem cell cycling and proper positioning within the subventricular zone niche via JAMC/RAP1Gap signaling
doi: 10.4103/1673-5374.339007
Figure Lengend Snippet: Primary and secondary antibodies used in this study
Article Snippet: Rat monoclonal anti- mouseVCAM1 ,
Techniques: